pKLy6.1-2R (ATCC® 63181)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: AL Bothwell

Permits and Restrictions

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Designations pKLy6.1-2R
GenBank Number

X04653

Species Mus musculus, mouse
Depositors AL Bothwell
Vector
Construct size (kb): 3.5
Insert
DNA: cDNA
Insert lengths(kb): 0.7609999775886536
Tissue: MethA fibrosarcoma cell line
Gene product: lymphocyte antigen-6 [DAG]
Alleles: 6.1, 6.1, 6.1, 6.2, 6.2, 6.2, 6.1, 6.1, 6.2, 6.2, 6.1, 6.2
Insert Size (kb) 0.761
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.3, 1.15; EcoRI--2.7, 0.8; PstI--3.2, 0.35; PvuII--2.4, 1.15; SacI--2.9, 0.3 (doublet).
The insert contains the following restriction sites (nt from the 5' end): SacI--160, 467; PstI--480.
Encodes Ly-6E.1 (Ly-6a allele) recognized by the SK70.94 and HD42 monoclonal antibodies.
Contains the complete coding sequence (nt 89-492) and extends from a linker-derived EcoRI site at the 5' end to a naturally occurring EcoRI site in the 3' untranslated region.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

LeClair KP, et al. Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J. 5: 3227-3234, 1986. PubMed: 3028776

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