liver 10 cDNA [liver 10] (ATCC® 63064)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: JH Weis

Permits and Restrictions

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Designations liver 10 cDNA [liver 10]
Species Mus musculus, mouse
Depositors JH Weis
Applications
When the 340 bp BamHI/BamHI fragment (lacking the extra 129 nucleotides) is used as a probe, the following EcoRI fragments are detected in BALB/c mice: 7.5 kb, 5.6 kb (chromosome 8), and 4.5 kb.
Vector
Construct size (kb): 6.150000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.781999945640564
Tissue: liver
Gene product: complement receptor related protein [Crry]
Insert Size (kb) 1.782
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; HindIII--5.6, 0.5; BamHI--4.3, 1.2, 0.3; PstI--6.0.
The insert contains the following sites (nucleotide position according to the published sequence): BamHI--514, 848; HindIII--1240, XbaI--1480.
Crry and Cr-2 are within 10 kb of each other and are transcribed in the same direction.
Encodes a single open reading frame starting at nt 482 of the published sequence and extending through the 3' end of the cDNA. Comparison with genomic sequence shows this clone lacks sequences for 7 additional amino acids.
Spleen 5 cDNA (ATCC 63065) differs from liver 10 cDNA (ATCC 63064) by having an additional 129 nucleotides between the signal sequence and the nucleotides encoding the first 60 aa repeat. Spleen 5 also does not extend as far in the 5' direction.
Liver 10 cDNA and spleen 5 cDNA are clones of alternative splice products of the Crry gene. The mRNAs are transcribed in all tissues examined in a 10 (liver 10) to 1 (spleen 5) ratio.
When the 340 bp BamHI/BamHI fragment (lacking the extra 129 nucleotides) is used as a probe, the following EcoRI fragments are detected in BALB/c mice: 7.5 kb, 5.6 kb (chromosome 8), and 4.5 kb.
Detects mRNA species of 1.9, 2.0, and 2.1 kb.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Paul MS, et al. The murine complement receptor gene family III: the genomic and transcriptional complexity of the Crry and Crry-ps genes. J. Immunol. 144: 1988-1996, 1990. PubMed: 2307848

Paul MS, et al. The murine complement receptor family. Analysis of mCRY gene products and their homology to human CR1. J. Immunol. 142: 582-589, 1989. PubMed: 2911011

Kingsmore SF, et al. Genetic organization of complement receptor-related genes in the mouse. J. Exp. Med. 169: 1479-1484, 1989. PubMed: 2564419

Utani A, et al. Mouse kalinin B1 (laminin beta 3 chain): cloning and tissue distribution. Lab. Invest. 72: 300-310, 1995. PubMed: 7898049

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