pMC5/04 [pMC5.04] (ATCC® 63038)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: BF Tack

Permits and Restrictions

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Designations pMC5/04 [pMC5.04]
GenBank Number

M15079

M15079

Species Mus musculus, mouse
Depositors BF Tack
Vector
Construct size (kb): 8.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 5.217000007629395
Tissue: liver
Gene product: hemolytic complement [C5]
Insert Size (kb) 5.217
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 2.5, 1.5, 0.4; HindIII--4.5, 2.4, 1.7; BamHI--2.9, 2.7, 2.3, 0.6; PstI--2.5, 2.3, 1.4, 1.4; SacI--8.4.
Insert contains the following sites separated by (bp): (V/I)- 9- NcoI- 648- KpnI- 116- BglII- 242- XhoI- 711- BglI- 28- HindIII- 390- BamHI- 38- EcoRV- 567- HpaI- 28- BamHI- 759- HindIII- 971- AccI- 710- (I/V). V=vector, I=insert.
Because of internal XhoI, HpaI and BamHI sites, the insert can not easily be isolated as one fragment.
Contains an open reading frame of 4920 bp (specifying 1640 amino acids) and 351 bp of 3' untranslated sequence.
At the amino acid level, there is significant homology between the mouse, rat, porcine, bovine and human C5.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Wetsel RA, et al. Primary structure of the fifth component of murine complement. Biochemistry 26: 737-743, 1987. PubMed: 2436653

Siracusa LD, et al. A molecular genetic linkage map of mouse chromosome 2. Genomics 6: 491-504, 1990. PubMed: 1970329

Brian F Tack, personal communication

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