CRI-R191 (ATCC® 61736)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Collaborative Research, Inc.

Permits and Restrictions

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Designations CRI-R191
Species Homo sapiens, human
Depositors Collaborative Research, Inc.
Vector
Construct size (kb): 44.5
Insert
DNA: genomic
Insert lengths(kb): 14.0
Tissue: liver
Gene product: DNA Segment, single copy [D8S26]
Alleles: A2, B1, B2, B3, B4, A1, A3
Insert Size (kb) 14.0
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--26.0, 7.4, 5.8, 3.8; EcoRI--20.0, 11.0, 8.6, 4.2, 1.3; XbaI--20.0, 15.0, 3.8, 3.1; KpnI--20.0, 17.0, 3.0, 1.4; HindIII--22.0, 7.8, 6.6, 5.0, 1.6.
The MspI and TaqI polymorphisms have a combined PIC value of 0.67 and a combined heterozygosity of 76%.
Enzyme(s) not detecting polymorphism: BglII, PstI, HindIII, EcoRI, RsaI, HincII (panel of 5 individuals).
References

Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

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