55.E [55E] (ATCC® 61376)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Mandel

Permits and Restrictions

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Designations 55.E [55E]
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: DNA Segment, single copy [DXS105]
Alleles: E1, E2
Insert Size (kb) 1.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; EcoRI/HindIII--2.7, 1.8; PstI--4.5; XbaI--4.5.
When combined with the TaqI RFLP detected by cX55.7, the heterozygosity at DXS105 is about 52%.
This probe was derived from cos55 and contains some repetitive sequences. Hybridization should be at 42C in 50% formamide and washes should be at 68C, 0.2X SSC.
References

Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485

Arveiler B, et al. Genetic mapping of the Xq27-q28 region: new RFLP markers useful for diagnostic applications in fragile-X and hemophilia-B families. Am. J. Hum. Genet. 42: 380-389, 1988. PubMed: 2893549

Jean Louis Mandel, personal communication

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