55.E [55E] (ATCC® 61376)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Mandel

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Designations 55.E [55E]
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: DNA Segment, single copy [DXS105]
Alleles: E1, E2
Insert Size (kb) 1.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; EcoRI/HindIII--2.7, 1.8; PstI--4.5; XbaI--4.5.
When combined with the TaqI RFLP detected by cX55.7, the heterozygosity at DXS105 is about 52%.
This probe was derived from cos55 and contains some repetitive sequences. Hybridization should be at 42C in 50% formamide and washes should be at 68C, 0.2X SSC.
References

Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485

Arveiler B, et al. Genetic mapping of the Xq27-q28 region: new RFLP markers useful for diagnostic applications in fragile-X and hemophilia-B families. Am. J. Hum. Genet. 42: 380-389, 1988. PubMed: 2893549

Jean Louis Mandel, personal communication