pV10RB [TRGV10, VgammaIII] (ATCC® 61219)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pV10RB [TRGV10, VgammaIII]
GenBank Number

X07206

Species Homo sapiens, human
Vector
Construct size (kb): 3.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 0.4000000059604645
Gene product: T cell receptor, gamma, variable region V10 [TCRGV10]
Insert Size (kb) 0.400
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.0; EcoRI/HindIII--2.6, 0.4; EcoRI--3.0; XbaI--3.0, PstI--3.0.
TCRGV10 is the unique member of the V gamma III gene subgroup.
The 5' end of the gene is available as pV10PR (ATCC 59598, 59599).
Subcloned from lambdaJM15. Contains the 3' region of TCRGV10. The BamHI site was not reconstituted during cloning.
References

Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713

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