pERT25 (ATCC® 59472)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: U Muller

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Designations pERT25
Species Homo sapiens, human
Depositors U Muller
Construct size (kb): 4.300000190734863
DNA: genomic
Insert lengths(kb): 0.5600000023841858
Gene product: DNA Segment, single copy [D18S11]
Alleles: A1, A2
Insert Size (kb) 0.560
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
HindIII+SalI releases an 0.7 kb fragment containing the 56 bp insert.
Restriction digests of the clone give the following sizes (kb): SalI--4.3; HindIII--4.3; HindIII/SalI--3.6, 0.68; EcoRI--4.3; PstI--4.3.
HindIII, PstI, TaqI, EcoRV, PvuII, XmnI, BstXI, BstNI detected polymorphisms in a population of 12 Caucasians.
The EcoRI polymorphism was detected in a population of 29 Caucasians and 8 members of an inbred Venezuelan pedigree. Co-dominant segregation was demonstrated in one Venezuelan pedigree.
Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI.

Kupke KG, Muller U. Parental origin of the extra chromosome in trisomy 18. Am. J. Hum. Genet. 45: 599-605, 1989. PubMed: 2577470

Accili D, et al. A new EcoRI polymorphism for the insulin receptor gene. Nucleic Acids Res. 17: 821, 1989. PubMed: 2563580

Muller U, et al. Highly polymorphic DNA sequences in the distal region of the long arm of human chromosome 18. Cytogenet. Cell Genet. 45: 16-20, 1987. PubMed: 2885151

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