pERT25 (ATCC® 59472)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: U Muller

Permits and Restrictions

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Designations pERT25
Species Homo sapiens, human
Depositors U Muller
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 0.5600000023841858
Gene product: DNA Segment, single copy [D18S11]
Alleles: A1, A2
Insert Size (kb) 0.560
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
HindIII+SalI releases an 0.7 kb fragment containing the 56 bp insert.
Restriction digests of the clone give the following sizes (kb): SalI--4.3; HindIII--4.3; HindIII/SalI--3.6, 0.68; EcoRI--4.3; PstI--4.3.
HindIII, PstI, TaqI, EcoRV, PvuII, XmnI, BstXI, BstNI detected polymorphisms in a population of 12 Caucasians.
The EcoRI polymorphism was detected in a population of 29 Caucasians and 8 members of an inbred Venezuelan pedigree. Co-dominant segregation was demonstrated in one Venezuelan pedigree.
Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI.
References

Kupke KG, Muller U. Parental origin of the extra chromosome in trisomy 18. Am. J. Hum. Genet. 45: 599-605, 1989. PubMed: 2577470

Accili D, et al. A new EcoRI polymorphism for the insulin receptor gene. Nucleic Acids Res. 17: 821, 1989. PubMed: 2563580

Muller U, et al. Highly polymorphic DNA sequences in the distal region of the long arm of human chromosome 18. Cytogenet. Cell Genet. 45: 16-20, 1987. PubMed: 2885151

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