pTHH55 (ATCC® 59270)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

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Designations pTHH55
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs observed under normal conditions of hybridization and washing.
Vector
Construct size (kb): 9.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 7.0
Gene product: DNA Segment, single copy [D15S27]
Alleles: A1, A2
Insert Size (kb) 7.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 3.0; EcoRI--9.4; HindIII--5.8, 3.85.
Codominant segregation demonstrated in 42 3-generation families.
RFLPs observed under normal conditions of hybridization and washing.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, RsaI, TaqI.
References

Holm T, et al. Isolation and mapping of polymorphic DNA sequence (pTHH55) on chromosome 15 [D15S27]. Nucleic Acids Res. 16: 10945, 1988. PubMed: 2905041

Nakamura Y, et al. A mapped set of DNA markers for human chromosome 15. Genomics 3: 342-346, 1988. PubMed: 3243548

Holm T, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH55) on chromosome 15 [D15S27]. Nucleic Acids Res. 16: 3117, 1988. PubMed: 2897110

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