pTHH55 (ATCC® 59270)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Designations pTHH55
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs observed under normal conditions of hybridization and washing.
Vector
Construct size (kb): 9.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 7.0
Gene product: DNA Segment, single copy [D15S27]
Alleles: A1, A2
Insert Size (kb) 7.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 3.0; EcoRI--9.4; HindIII--5.8, 3.85.
Codominant segregation demonstrated in 42 3-generation families.
RFLPs observed under normal conditions of hybridization and washing.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, RsaI, TaqI.
References

Holm T, et al. Isolation and mapping of polymorphic DNA sequence (pTHH55) on chromosome 15 [D15S27]. Nucleic Acids Res. 16: 10945, 1988. PubMed: 2905041

Nakamura Y, et al. A mapped set of DNA markers for human chromosome 15. Genomics 3: 342-346, 1988. PubMed: 3243548

Holm T, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH55) on chromosome 15 [D15S27]. Nucleic Acids Res. 16: 3117, 1988. PubMed: 2897110

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