pGS14-10 (ATCC® 59249)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

View Permits

Designations pGS14-10
Species Homo sapiens, human
Applications
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes.
Vector
Construct size (kb): 12.39999961853027
Insert
DNA: genomic
Insert lengths(kb): 10.0
Tissue: HeLa cell line
Gene product: ribosomal protein S14 [RPS14]
Insert Size (kb) 10.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.8, 2.8; EcoRI--3.8, 3.2, 3.0, 2.3; PstI--4.8, 3.2, 2.3, 1.8; SalI--13.0; BamHI--4.6, 4.1, 3.8.
The insert extends from an EcoRI site to the HindIII site (about 1.5 to 11.5 kb). It includes 3 copies of a 10 bp sequence (-69, -29, +1) found in other ribosomal protein genes.
The coding sequence starts in exon 2. Introns 3 and 4 contain Alu-homologous repeats. The 3' flanking sequence contains 10 bp complementary to U1 RNA.
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes. It shows only slight cross-reaction with CHO cell (Chinese hamster) DNA.
The fragment cloned was an EcoRI/HindIII fragment to which a HindIII linker was added. Thus the clone contains both EcoRI and HindIII sites at its 5' end and a HindIII site at the 3' end. There are several internal EcoRI sites.
References

Diaz JJ, Roufa DJ. Fine-structure map of the human ribosomal protein gene RPS14. Mol. Cell. Biol. 12: 1680-1686, 1992. PubMed: 1549121

Rhoads DD, et al. Primary structure of human ribosomal protein S14 and the gene that encodes it. Mol. Cell. Biol. 6: 2774-2783, 1986. PubMed: 3785212

Product Sheet
Product Sheet
Product Sheet