pGS14-10 (ATCC® 59249)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Designations pGS14-10
Species Homo sapiens, human
Applications
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes.
Vector
Construct size (kb): 12.39999961853027
Insert
DNA: genomic
Insert lengths(kb): 10.0
Tissue: HeLa cell line
Gene product: ribosomal protein S14 [RPS14]
Insert Size (kb) 10.0
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.8, 2.8; EcoRI--3.8, 3.2, 3.0, 2.3; PstI--4.8, 3.2, 2.3, 1.8; SalI--13.0; BamHI--4.6, 4.1, 3.8.
The insert extends from an EcoRI site to the HindIII site (about 1.5 to 11.5 kb). It includes 3 copies of a 10 bp sequence (-69, -29, +1) found in other ribosomal protein genes.
The coding sequence starts in exon 2. Introns 3 and 4 contain Alu-homologous repeats. The 3' flanking sequence contains 10 bp complementary to U1 RNA.
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes. It shows only slight cross-reaction with CHO cell (Chinese hamster) DNA.
The fragment cloned was an EcoRI/HindIII fragment to which a HindIII linker was added. Thus the clone contains both EcoRI and HindIII sites at its 5' end and a HindIII site at the 3' end. There are several internal EcoRI sites.
References

Diaz JJ, Roufa DJ. Fine-structure map of the human ribosomal protein gene RPS14. Mol. Cell. Biol. 12: 1680-1686, 1992. PubMed: 1549121

Rhoads DD, et al. Primary structure of human ribosomal protein S14 and the gene that encodes it. Mol. Cell. Biol. 6: 2774-2783, 1986. PubMed: 3785212

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