phi6-3 (ATCC® 59134)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Litt

Permits and Restrictions

View Permits

Designations phi6-3
Species Homo sapiens, human
Depositors M Litt
Applications
Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe.
Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 14.5
Gene product: DNA Segment, single copy [D11S85]
Alleles: A1, B2, A2, B1
Insert Size (kb) 14.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0.
phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
References

Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085

Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469

Maslen CL, et al. Seven polymorphic loci mapping to human chromosomal region 11q22-qter. Genomics 2: 66-75, 1988. PubMed: 2898428

Michael Litt, personal communication