phi6-3 (ATCC® 59134)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Litt

Permits and Restrictions

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Designations phi6-3
Species Homo sapiens, human
Depositors M Litt
Applications
Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe.
Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 14.5
Gene product: DNA Segment, single copy [D11S85]
Alleles: A1, B2, A2, B1
Insert Size (kb) 14.5
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0.
phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
References

Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085

Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469

Maslen CL, et al. Seven polymorphic loci mapping to human chromosomal region 11q22-qter. Genomics 2: 66-75, 1988. PubMed: 2898428

Michael Litt, personal communication

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