CHB205 (ATCC® 57072)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

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Designations CHB205
GenBank Number

J00138

J00138

Species Homo sapiens, human
Vector
Construct size (kb): 6.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 1.649999976158142
Tissue: fetal liver
Gene product: dihydrofolate reductase [DHFR]
Alleles: A1, A2
Insert Size (kb) 1.650
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.5; SalI--5.5; PvuII--5.5; PstI--5.5; HindIII--5.5; EcoRI--4.4, 1.7.
Insert contains these sites in kb from 5' EcoRI site: SstI--0.04; XbaI--0.06; AvaI/SstI--0.5; BglII--1.0; XhoI--1.2; EcoRI--1.65.
The insert includes exon 4 and part of intron 4.
References

Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421

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