CHB203 [pCS806] (ATCC® 57068)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: T Shimada

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Designations CHB203 [pCS806]
GenBank Number

J00139

Species Homo sapiens, human
Depositors T Shimada
Vector
Construct size (kb): 13.0
Insert
DNA: genomic
Insert lengths(kb): 8.600000381469727
Tissue: fetal liver
Gene product: dihydrofolate reductase [DHFR]
Alleles: A1, A2
Insert Size (kb) 8.600
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.5, 4.0, 3.4; SalI--14.0; PvuII--6.2, 4.2, 2.75; EcoRI--8.6, 4.4; HindIII--9.4, 3.7.
Insert contains these sites in kb from 5' end: BglII--0.03, 3.3; AvaI--1.8; BglI--2.2, 7.5; SmaI--2.3; HindIII--3.5; PstI--4.6, 6.5, 7.3; XbaI--4.7; BamHI--5.0, 8.2; XbaI--6.3; SmaI/AvaI--7.2, 7.7; EcoRI--8.6.
The insert includes exon 6 in a 1.2 kb SmaI/HindIII fragment.
References

Anagnou NP, et al. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81: 5170-5174, 1984. PubMed: 6089182

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421

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