CHB203 [pCS806] (ATCC® 57068)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: T Shimada

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Designations CHB203 [pCS806]
GenBank Number

J00139

Species Homo sapiens, human
Depositors T Shimada
Vector
Construct size (kb): 13.0
Insert
DNA: genomic
Insert lengths(kb): 8.600000381469727
Tissue: fetal liver
Gene product: dihydrofolate reductase [DHFR]
Alleles: A1, A2
Insert Size (kb) 8.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.5, 4.0, 3.4; SalI--14.0; PvuII--6.2, 4.2, 2.75; EcoRI--8.6, 4.4; HindIII--9.4, 3.7.
Insert contains these sites in kb from 5' end: BglII--0.03, 3.3; AvaI--1.8; BglI--2.2, 7.5; SmaI--2.3; HindIII--3.5; PstI--4.6, 6.5, 7.3; XbaI--4.7; BamHI--5.0, 8.2; XbaI--6.3; SmaI/AvaI--7.2, 7.7; EcoRI--8.6.
The insert includes exon 6 in a 1.2 kb SmaI/HindIII fragment.
References

Anagnou NP, et al. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81: 5170-5174, 1984. PubMed: 6089182

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421

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