pGL2C (53367)

Applications: expression vector  /  Depositors: Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Permits and Restrictions

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Designations pGL2C
Depositors Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli JM83; K-12 strain JM83
Vector Information
Size (kb): 6.5999999046325680
Vector: pGL2C (plasmid)
Promoters: Promoter glucoamylase
Construction: pUC12
Marker(s):ampR
Construct size (kb): 6.599999904632568
Features: marker(s): ampR
promoter: glucoamylase
replicon: pMB1
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; BglII--7.0, EcoRI--4.6, 1.8, 0.33.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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