Monosiga gracilis Kent (ATCC® 50964)

Organism: Monosiga gracilis Kent  /  Depositor: TA Nerad

Strain Designations BSA-02190019
Biosafety Level 1
Isolation
salt marsh sediment, Cattleshed Marsh, eastern shore of Virginia, 2000
Product Format frozen
Type Strain no
Medium Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Klebsiella pneumoniae subsp. pneumoniae ATCC 700831 and mixed bacteria
Protocol: To subcultivate, virgorously agitate a T-25 flask at or near peak density and aseptically transfer a 0.5-ml aliquot to a fresh T-25 flask containing 10 ml of bacterized ATCC medium 1525.
Subcultivation
Protocol: To subcultivate, virgorously agitate a T-25 flask at or near peak density and aseptically transfer a 0.5-ml aliquot to a fresh T-25 flask containing 10 ml of bacterized ATCC medium 1525.
Name of Depositor TA Nerad
Year of Origin 2000
References

Thomas A Nerad, personal communication