Naegleria sp. (ATCC® 50349)

Organism: Naegleria sp.  /  Depositor: BS Robinson

Strain Designations SWL NG-206
Biosafety Level 1
Isolation
freshwater, River Murray, Mannun, Australia, 1984
Product Format freeze-dried
Type Strain no
Comments
Zymodeme Gr-1
Equals NG-206, maximum temperature tolerance 40C
This strain and ATCC 50352 are members of Naegleria sp. genetic cluster H
Medium Medium 997: Fresh water ameba medium
Growth Conditions
Max Temperature: 30.0°C
Min Temperature: 25.0°C
Cryopreservation

1.   Allow the cells to encyst.  To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Page's Balanced Salt Solution).  Rub the surface of the plate with a spread bar to detach adhering amoebae.

2.   Transfer the cyst suspension to a sterile centrifuge tube.

3.   If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration.  To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106.

4.   While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:  Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.  Allow the DMSO to solidify.  Add the required volume of refrigerated medium.  Dissolve the DMSO by inverting the tube several times. 

Name of Depositor BS Robinson
Year of Origin 1984
References

Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158

Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.