Singhamoeba horticola (Singh and Hanumaiah) Sawyer et al. (ATCC® 50197)

Organism: Singhamoeba horticola (Singh and Hanumaiah) Sawyer et al.  /  Depositor: TK Sawyer

Deposited As Schizopyrenus horticolus Singh and Hanumaiah
Strain Designations CR-1-3
Biosafety Level 1
Isolation
bottom sediment, Chester River, MD, 1985
Product Format dried
Type Strain no
Comments
species description
Medium Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Name of Depositor TK Sawyer
Year of Origin 1985
References

Sawyer TK, et al. Singhamoeba horticala new combination Singh and Hanumaiah 1979 type species of Singhamoeba new genus. J. Protozool. 39: 107-109, 1992.