pDSV100 (ATCC® 45118)

Organism: Digitaria streak virus  /  Clone Type: Clone  /  Depositors: PM Mullineaux, GP Accotto

Permits and Restrictions

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Designations pDSV100
GenBank Number

M23022

Species Digitaria streak virus
Depositors PM Mullineaux, GP Accotto
Vector
Construct size (kb): 3.98799991607666
Insert
DNA: cDNA
Insert lengths(kb): 0.8799999952316284
Gene product: coat protein V2
Insert Size (kb) 0.880
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SacI--4.0; HindIII--4.0; SalI--4.0; PstI--3.3, 0.7.
The insert contains the following restriction sites (nt position according the published sequence): KpnI--249, 674, 1008; PstI--454; SacI--506; NruI--883.
Linearizing with SphI or HindIII and transcription with SP6 RNA polymerase will give the sense strand.
The SP6-derived transcript would direct the translation of the V2 (coat protein) ORF in an in vitro translation system, such as wheat germ or rabbit reticulocyte lysate.
Constructed from the MluI/SpeI fragment extending from nt 183 to 1071 of the published sequence. The EcoRI/MluI and SpeI/XbaI sites were destroyed.
Classification Geminivirus
References

Donson J, et al. The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology 161: 160-169, 1987. PubMed: 3672928

P M Mullineaux, personal communication

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