pDSV100 (ATCC® 45118)

Organism: Digitaria streak virus  /  Clone Type: Clone  /  Depositors: PM Mullineaux, GP Accotto

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Designations pDSV100
GenBank Number

M23022

Species Digitaria streak virus
Depositors PM Mullineaux, GP Accotto
Vector
Construct size (kb): 3.98799991607666
Insert
DNA: cDNA
Insert lengths(kb): 0.8799999952316284
Gene product: coat protein V2
Insert Size (kb) 0.880
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): SacI--4.0; HindIII--4.0; SalI--4.0; PstI--3.3, 0.7.
The insert contains the following restriction sites (nt position according the published sequence): KpnI--249, 674, 1008; PstI--454; SacI--506; NruI--883.
Linearizing with SphI or HindIII and transcription with SP6 RNA polymerase will give the sense strand.
The SP6-derived transcript would direct the translation of the V2 (coat protein) ORF in an in vitro translation system, such as wheat germ or rabbit reticulocyte lysate.
Constructed from the MluI/SpeI fragment extending from nt 183 to 1071 of the published sequence. The EcoRI/MluI and SpeI/XbaI sites were destroyed.
Classification Geminivirus
References

Donson J, et al. The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology 161: 160-169, 1987. PubMed: 3672928

P M Mullineaux, personal communication

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