pbc-N1 (ATCC® 41001)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Barbacid

Permits and Restrictions

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Designations pbc-N1
GenBank Number

J00277

Species Homo sapiens, human
Depositors M Barbacid
Vector
Construct size (kb): 10.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 6.400000095367432
Tissue: fetal liver
Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]
Alleles: F1, F2, F3, C1, F1, F2, F3, C1, H4, H4, H1, H2, H3, D1, D2, H1, H2, H3, D1, D2, C2, C2
Insert Size (kb) 6.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains SacI (3), PvuII (2), XhoI, XbaI, KpnI, SphI, ClaI, and BglII sites.
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--6.4, 4.4; XhoI--11.0; EcoRI--11.0; BglII--11.0.
This is closely related to the T24 oncogene. There is a 200 bp deletion in this sequence between the SphI and ClaI sites.
This is not the transforming sequence. It differs from the transforming gene at 2 nucleotides, one of which is coding.
References

Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3' to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828

Pierotti MA, et al. Two RFLPs generated by TaqI at the human HRAS1 locus. Nucleic Acids Res. 14: 4379, 1986. PubMed: 3012470

Pulciani S, et al. Oncogenes in human tumor cell lines: Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc. Natl. Acad. Sci. USA 79: 2845-2849, 1982. PubMed: 6953433

Santos E, et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298: 343-347, 1982. PubMed: 6283384

Reddy EP. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science 220: 1061-1063, 1983. PubMed: 6844927

Pulciani S, et al. Transforming genes in human tumors. J. Cell. Biochem. 20: 51-61, 1982. PubMed: 6761348

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