pbc-N1 (ATCC® 41001)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Barbacid

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Designations pbc-N1
GenBank Number

J00277

J00277

Species Homo sapiens, human
Depositors M Barbacid
Vector
Construct size (kb): 10.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 6.400000095367432
Tissue: fetal liver
Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]
Alleles: F1, F2, F3, C1, F1, F2, F3, C1, H4, H4, H1, H2, H3, D1, D2, H1, H2, H3, D1, D2, C2, C2
Insert Size (kb) 6.400
Biosafety Level 1
Comments
The insert contains SacI (3), PvuII (2), XhoI, XbaI, KpnI, SphI, ClaI, and BglII sites.
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--6.4, 4.4; XhoI--11.0; EcoRI--11.0; BglII--11.0.
This is closely related to the T24 oncogene. There is a 200 bp deletion in this sequence between the SphI and ClaI sites.
This is not the transforming sequence. It differs from the transforming gene at 2 nucleotides, one of which is coding.
References

Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3' to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828

Pierotti MA, et al. Two RFLPs generated by TaqI at the human HRAS1 locus. Nucleic Acids Res. 14: 4379, 1986. PubMed: 3012470

Pulciani S, et al. Oncogenes in human tumor cell lines: Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc. Natl. Acad. Sci. USA 79: 2845-2849, 1982. PubMed: 6953433

Santos E, et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298: 343-347, 1982. PubMed: 6283384

Reddy EP. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science 220: 1061-1063, 1983. PubMed: 6844927

Pulciani S, et al. Transforming genes in human tumors. J. Cell. Biochem. 20: 51-61, 1982. PubMed: 6761348

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