Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux (ATCC® 40615)

Organism: Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux  /  Depositor: JK Frenkel

Deposited As Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Strain Designations T-263
Application
Food and waterborne pathogen research
Opportunistic pathogen research
Biosafety Level 2
Isolation
Mutant clone derived from the adenine arabinoside-resistant mutant of the cat ('C') strain, ?.
Product Format frozen
Type Strain no
Comments
Immunization of cats against Toxoplasmosis
Growth Conditions
Duration: in vivo, mouse
Protocol: ATCCNO: 40615 SPEC: Thaw the frozen ampule using normal protocol and divide into 6 equal aliquots. Inject each aliquot subcutaneously into a 6- to 9-week-old mouse. For use as a vaccine, for the infection of cats, and for storage in animals, Toxoplasma must be inoculated into mice that are treated with sodium sulfadiazine, 15 milligrams per 100 ml of drinking water. This will permit the development of chronic infection and the production of bradyzoites necessary to infect and immunize cats. This strain should not be maintained in cell culture because the formation of bradyzoites will be impaired after 10 to 20 passages and may disappear after 30 passages.
Subcultivation
Protocol: ATCCNO: 40615 SPEC: Thaw the frozen ampule using normal protocol and divide into 6 equal aliquots. Inject each aliquot subcutaneously into a 6- to 9-week-old mouse. For use as a vaccine, for the infection of cats, and for storage in animals, Toxoplasma must be inoculated into mice that are treated with sodium sulfadiazine, 15 milligrams per 100 ml of drinking water. This will permit the development of chronic infection and the production of bradyzoites necessary to infect and immunize cats. This strain should not be maintained in cell culture because the formation of bradyzoites will be impaired after 10 to 20 passages and may disappear after 30 passages.
Name of Depositor JK Frenkel
References

Frenke JK, Pfeffekorn ER. Vaccine for immunizing cats against Toxoplasma oocyst shedding. US Patent 5,045,313 dated Sep 3 1991