HPV 56 clone 2C purified plasmid DNA (ATCC® 40549)

Organism: human papilloma virus type 56  /  Clone Type: Clone  /  Depositors: Life Technologies, Inc., AT Lorincz, Life Technologies, Inc.

Designations HPV 56 clone 2C purified plasmid DNA
Species human papilloma virus type 56
Depositors Life Technologies, Inc., AT Lorincz, Life Technologies, Inc.
Vector Total size of construct (kb): 10.7
Marker: ampR
Excise insert: BamHI

Vector: pT713
Vector type: plasmid
Vector size (kb): 2.82
Markers: ampR
Vector ends: BamHI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI XmaI SstI EcoRI
Construction: pBR322, pUC13
Promter: T7 (phi10)
Insert Insert lengths (kb): 7.9
Gene: human papilloma virus type 56
DNA: genomic
Source: from human cervical intraepithelial neoplasia I
Insert ends: BamHI
Biosafety Level 1
Shipping Information Purified plasmid DNA, 200 ng dried
Comments
The following enzymes do not cut the insert: BglI, HindIII, PvuI, SalI, SmaI, SstI, XhoI.
The insert contains the following restriction sites (in approximate kb from one end): EcoRV--1.8; EcoRI--3.1; EcoRV--3.2; PvuII--4.8, XbaI--4.92; SphI--5.2; EcoRI--5.9; PstI--6.65; PstI/KpnI--7.1; BamHI--0/7.9.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 3.1, 2.8; BamHI--7.9, 3.0.
Partial sequence cloned from human cervical intraepithelial neoplasia I
Classification Papovavirus, Papillomavirus, recombinant
References

Lorincz AT. Human papillomavirus 56 nucleic acid hybridization probes and methods for employing the same. US Patent 4,908,306 dated Mar 13 1990

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