pTPA25 [BG87] (ATCC® 39808)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Biogen, Inc., R Fisher, Biogen, Inc.

Permits and Restrictions

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Designations pTPA25 [BG87]
GenBank Number

X02901

Species Homo sapiens, human
Depositors Biogen, Inc., R Fisher, Biogen, Inc.
Applications
contains sequence plasminogen activator, tissue
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PLAT
Genomic copy number: unique
Gene name: plasminogen activator, tissue
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p12-q11.2
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: BamHI
Insert size (kb): 3.0
Cross references: DNA Seq. Acc.: X02901
Insert lengths(kb): 3.0
Gene product: plasminogen activator, tissue [PLAT]
Target Gene: plasminogen activator, tissue
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; BamHI/HindIII--3.5, 3.1; HindIII--6.6.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.25, 2.1; EcoRI--0.7, 1.2; PstI--0.55, 0.6, 1.25, 1.3; PvuII--1.25, 1.3, 1.6; SacI--0.05, 1.35; SmaI--1.65; StuI--1.4.
The 3' end of the insert contains the SV40 small t antigen splice site.
References

Wallner BP, et al. DNA sequences, recombinant DNA molecules and processes for producing human phospholipase inhibitor-like polypeptides. US Patent 4,874,743 dated Oct 17 1989

Product Sheet
Product Sheet
Product Sheet