pTPA25 [BG87] (39808)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Biogen, Inc., R Fisher, Biogen, Inc.

Permits and Restrictions

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Designations pTPA25 [BG87]
GenBank Number

X02901

Species Homo sapiens, human
Depositors Biogen, Inc., R Fisher, Biogen, Inc.
Applications
contains sequence plasminogen activator, tissue
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PLAT
Genomic copy number: unique
Gene name: plasminogen activator, tissue
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p12-q11.2
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: BamHI
Insert size (kb): 3.0
Cross references: DNA Seq. Acc.: X02901
Insert lengths(kb): 3.0
Gene product: plasminogen activator, tissue [PLAT]
Target Gene: plasminogen activator, tissue
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; BamHI/HindIII--3.5, 3.1; HindIII--6.6.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.25, 2.1; EcoRI--0.7, 1.2; PstI--0.55, 0.6, 1.25, 1.3; PvuII--1.25, 1.3, 1.6; SacI--0.05, 1.35; SmaI--1.65; StuI--1.4.
The 3' end of the insert contains the SV40 small t antigen splice site.
References

Wallner BP, et al. DNA sequences, recombinant DNA molecules and processes for producing human phospholipase inhibitor-like polypeptides. US Patent 4,874,743 dated Oct 17 1989

Product Sheet
Product Sheet
Product Sheet