pMDIII (ATCC® 37502)

Applications: expression vectorshuttle vector  /  Depositors: H Wolf

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Designations pMDIII
Depositors H Wolf
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0000000000000000
Vector: pMDIII (plasmid)
Promoters: Promoter SV40 early
Marker(s):ampR
Construct size (kb): 7.0
Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
enhancer: SV40
Applications
expression vector
shuttle vector
Comments
A vector for expression of cloned sequences under the control of the SV40 early promoter in mammalian cell culture selected for dhfr+ phenotype.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Breathnach R, Harris BA. Plasmids for the cloning and expression of full-length double-stranded cDNAs under the control of the SV40 early or late gene promoter. Nucleic Acids Res. 11: 7119-7136, 1983. PubMed: 6314276

Kaufman RJ, Sharp PA. Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression. Mol. Cell. Biol. 2: 1304-1319, 1982. PubMed: 6131378

Motz M, et al. Expression of the Epstein-Barr virus major membrane protein in Chinese hamster ovary cells. Gene 44: 353-359, 1986. PubMed: 3023198

Shipped freeze-dried