pEMBLYi21 (ATCC® 37398)

Applications: YI-type (integrating) shuttle vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: G Cesareni

Permits and Restrictions

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Designations pEMBLYi21
Depositors G Cesareni
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.0999999046325680
Vector: pEMBLYi21 (phagemid)
Construction: pEMBL9, (ATCC 37395), bacteriophage f1, URA3
Marker(s):URA3,ampR
Construct size (kb): 5.099999904632568
Features: insert detection: lacZ'
marker(s): ampR, URA3
replicon: pMB1, f1
Applications
YI-type (integrating) shuttle vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
ss-DNA-producing plasmid with polylinker in lacZ' and marker selectable in yeast.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771

Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935

Shipped freeze-dried