pICEM19H- (ATCC® 37378)

Applications: vector permitting production of single-stranded DNA  /  Depositors: J Marsh

Permits and Restrictions

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Designations pICEM19H-
Depositors J Marsh
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.9730000495910650
Vector: pICEM19H- (phagemid)
Construction: pIC7, pEMBL8-
Construct size (kb): 3.973000049591065
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
vector permitting production of single-stranded DNA
Streak to xgal + amp plate and pick a blue colony.
Phagemid with polylinker in lacZ' for production of ssDNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Marsh JL, et al. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481-485, 1984. PubMed: 6099327

Shipped freeze-dried