ptac12 (ATCC® 37138)

Applications: contains easily purifiable cassette(s) for constructionexpression vector  /  Depositors: E Amann

Permits and Restrictions

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Designations ptac12
Depositors E Amann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.60
Vector: ptac12 (plasmid)
Promoters: Promoter tac
Construction: pEA300 (ATCC 37181), pGL101
Construct size (kb): 2.6
Features: marker(s): ampR
promoter: tac
replicon: pMB1
enhancer: none
contains easily purifiable cassette(s) for construction
expression vector
A 55 bp HpaII/PvuII fragment containing the -10 region of the lacUV5 promoter was inserted into the ClaI site of pEA300 (ATCC 37181).
Contains the lac operator. The PvuII site is located 5 bp downstream of the lacZ ribosome binding sequence.
The tac promoter can be excised by a EcoRI/PvuII digest.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1982.

Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.