pBR325Amy (ATCC® 31792)

Organism: Geobacillus stearothermophilus (Donk) Nazina et al.  /  Clone Type: Clone  /  Depositors: CPC International, Inc.

Permits and Restrictions

View Permits

Designations pBR325Amy
Species Geobacillus stearothermophilus (Donk) Nazina et al.
Depositors CPC International, Inc.
Applications
produces protein amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Vector
Construct size (kb): 11.19999980926514
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references:
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references:
Insert lengths(kb): 5.400000095367432
Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A)
Target Gene: amylase, alpha 1A; salivary
Insert Size (kb) 5.400
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Plasmid expresses a thermostable alpha-amylase.
References

Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985

Product Sheet
Product Sheet
Product Sheet