Capsaspora owczarzaki Hertel et al. (ATCC® 30864)

Organism: Capsaspora owczarzaki Hertel et al.  /  Depositor: CJ Bayne

Permits and Restrictions

View Permits

Deposited As Nuclearia sp.
Strain Designations No designation
Application
Renamed as Capsaspora owczarzaki based on SSU rRNA analysis (Hertel, LA, et al.
Biosafety Level 1
Isolation Biomphalaria glabrata, Corvallis, OR, 1977
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Genome Sequenced Strain

Yes

Comments
Originally deposited as Nuclearia sp. Renamed as Capsaspora owczarzaki based on SSU rRNA analysis (Hertel, LA, et al. 2006 Int J Parasitol. 32:1183-1191).
Destroys Schistosoma mansoni sporocysts in vitro
Genome sequencing strain (Broad Institute)
Medium ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
ATCC® Medium 803: M7 medium
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 106/mL.  If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 20% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:  Add the required volume of DMSO to a glass screw-capped test tube and place on ice.  Allow the DMSO to solidify.  Add the required volume of refrigerated ATCC medium 1034.  Dissolve the DMSO by inverting several times.  If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 mL of fresh ATCC medium 1034.
  10. Incubate the flask horizontally at 25°C with the cap screwed on tightly.
Name of Depositor CJ Bayne
Chain of Custody
ATCC <-- CJ Bayne <-- HH Stibbs
Year of Origin 1977
References

Owczarzak A, et al. The destruction of Schistosoma mansoni mother sporocysts in vitro by amoebae isolated from Biomphalaria glabrata: an ultrastructural study. J. Invertebr. Pathol. 35: 26-33, 1980. PubMed: 7365267

Stibbs HH, et al. Schistosome sporocyst-killing amoebae isolated from Biomphalaria glabrata. J. Invertebr. Pathol. 33: 159-170, 1979. PubMed: 501126

Hertel LA, et al. The symbiont Capsaspora owczarzaki, nov. gen. nov. sp., isolated from three strains of the pulmonate snail Biomphalaria glabrata is related to members of the Mesomycetozoea. Int. J. Parasitol. 32: 1183-1191, 2002. PubMed: 12117501

Amaral-Zettler LA, et al. The Nucleariid Amoebae: More Protists at the Animal-Fungal Boundary. J Eukaryot Microbiol. 48:293-297, 2001. PubMed: 11411837.

Ruiz-Trillo, I, et al. Insights into the evolutionary origin and genome architecture of the unicellular opisthokonts Capsaspora owczarzaki and Sphaeroforma arctica. J Eukaryot Microbiol. 53:379-384, 2006. PubMed: 16968456.

Suga H, et al. The Capsaspora genome reveals a complex unicellular prehistory of animals. Nat Commun 4: 2325, 2013. PubMed: 23942320

Cross References

Nucleotide (GenBank) : ACFS01000000 Capsaspora owczarzaki ATCC 30864, whole genome shotgun sequencing project.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation