Vahlkampfia lobospinosa (Craig) Craig (ATCC® 30298)

Organism: Vahlkampfia lobospinosa (Craig) Craig  /  Depositor: WE Wilhelm, ER Fizer

Permits and Restrictions

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Application
Restriction endonuclease analysis of mitochondrial DNA
Biosafety Level 1
Isolation
cattle feces, Shelby County, TN, 1969
Product Format dried
Type Strain no
Comments
Circular ribosomal RNA genes
biochemical identification
Restriction endonuclease analysis of mitochondrial DNA
Identification and phylogenetic relationships
Promitosis
use of plastic ampoules for freeze preservation
Growth Conditions
Temperature: 35.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Name of Depositor WE Wilhelm, ER Fizer
Year of Origin 1969
References

Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593

Milligan SM, Band RN. Restriction endonuclease analysis of mitochondrial DNA as an aid in the taxonomy of Naegleria and Vahlkampfia. J. Protozool. 35: 198-204, 1988. PubMed: 2840491

Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492

Wilhelm WE, Anderson JH. Vahlkampfia lobospinosa (Craig. 1912) Craig. 1913: rediscovery of a coprozoic ameba. J. Parasitol. 57: 1378-1379, 1971. PubMed: 5157177

Brown S, De Jonckheere JF. Identification and phylogenetic relationships of Vahlkampfia spp. (free-living amoebae) by riboprinting. FEMS Microbiol. Lett. 115: 241-246, 1994.

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Fizer ER, Wilhelm WE. Promitosis and rod occurrence in the limax ameba Vahlkampfia lobospinosa. J. Protozool. 25: 34-38, 1978. PubMed: 660570

Cross References

Nucleotide (GenBank) : M98052 Vahlkampfia lobospinosa 16S ribosoma RNA-like gene.

Nucleotide (GenBank) : M98052 Vahlkampfia lobospinosa 16S ribosoma RNA-like gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation