Polytomella caeca Pringsheim (ATCC® 30295)

Depositor: DL Wise  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Biosafety Level 1
Product Format freeze-dried
Type Strain no
Comments
Preservation
Life cycle
Medium Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Subcultivation
Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Name of Depositor DL Wise
Chain of Custody
ATCC <<--DL Wise<<--UTEX 963 <<--- E.G. Pringsheim -- (?)
References

Reed RB, et al. Preservation of encysted Polytomella. J. Gen. Microbiol. 97: 29-34, 1976. PubMed: 186558

Lewis E, et al. Life cycle of Polytomella caeca (phytomonadida, Polyblepharidae). J. Protozool. 21: 647-649, 1974.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation