Blastocrithidia culicis (Novy et al.) Wallace and Johnson (ATCC® 30257)

Strain Designations: adiplosomic  /  Depositor: FG Wallace  /  Biosafety Level: 1

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Strain Designations adiplosomic
Biosafety Level 1
Isolation
mosquito, Aedes vexans, Mound, MN, 1952
Product Format frozen
Type Strain no
Comments
nutritional requirements
Urea cycle in wild aposymbiotic strains
isoenzyme electrophoresis for species identification
Trypomastigotes in cultures
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Max Temperature: 25.0°C
Min Temperature: 21.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1952
References

De Menezes MC, Roitman I. Nutritional requirements of Blastocrithidia culicis, a trypanosomatid with an endosymbiont. J. Protozool. 38: 122-123, 1991.

Wallace and Johnson. . J. Insect Pathol. 3: 75-80, 1961.

Eberhard ML, et al. Development of Dipetalonema gracile and D. caudispina to the infective stage in Culicoides hollensis. J. Parasitol. 65: 89-95, 1979. PubMed: 571910

Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788

de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation