Plasmodium hylobati Rhodain ATCC ® 30154™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	WAK
Application	Vector borne research
Biosafety Level	2
Isolation	Hylobates molock, Sarawak, 1968
Product Format	frozen
Type Strain	no

Growth Conditions	Duration: in vivo, Macaca mulatta Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation	Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).

Growth Conditions

Duration: in vivo, Macaca mulatta

Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).

Subcultivation

Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).

Name of Depositor	National Institutes of Health
Year of Origin	1968
References	Collins WE, et al. Plasmodium hylobati: a malaria parasite of the gibbon. J. Parasitol. 58: 123-128, 1972. PubMed: 4335047 Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971. Sodeman TM, et al. Studies of the exoerythrocytic stages of simian malaria. VI. Plasmodium hylobati. J. Parasitol. 58: 129-134, 1972. PubMed: 4335048

Name of Depositor

National Institutes of Health

Year of Origin

1968

References

Collins WE, et al. Plasmodium hylobati: a malaria parasite of the gibbon. J. Parasitol. 58: 123-128, 1972. PubMed: 4335047

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

Sodeman TM, et al. Studies of the exoerythrocytic stages of simian malaria. VI. Plasmodium hylobati. J. Parasitol. 58: 129-134, 1972. PubMed: 4335048

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Plasmodium hylobati Rhodain (ATCC® 30154™)

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Plasmodium hylobati Rhodain (ATCC^® 30154^™)