Plasmodium berghei Vincke and Lips (ATCC® 30090)

Strain Designations: NK65  /  Depositor: M Yoeli  /  Biosafety Level: 1

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Deposited As Plasmodium berghei berghei Vincke and Lips
Strain Designations NK65
Application
Vector borne research
Biosafety Level 1
Isolation
mosquito, Anopheles dureni, Forest Gallery of Kisanga, Katanga, 1965
Product Format frozen
Type Strain no
Comments
Suitable for cyclical transmission in Anopheles stephensis.
Gametogony and sporogony
Growth Conditions
Duration: in vivo, hamster
Protocol: ATCCNO: 30090 SPEC: Serial passage in young hamsters (for best results) or laboratory mice. Inject 0.2-0.5 ml of an ampule intraperitoneally into 2 or 3 young hamsters (6-8 weeks old). Gametocytes develop best in hamsters. Inject 0.2-0.5 ml from the heart for serial passage.
Subcultivation
Protocol: ATCCNO: 30090 SPEC: Serial passage in young hamsters (for best results) or laboratory mice. Inject 0.2-0.5 ml of an ampule intraperitoneally into 2 or 3 young hamsters (6-8 weeks old). Gametocytes develop best in hamsters. Inject 0.2-0.5 ml from the heart for serial passage.
Cryopreservation

CRYOPRESERVATION: 

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

1.   To harvest parasites, inject host with ketamine (0.1–0.2 ml).

2.   Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaeger's anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.                                                                                                                                                             Glass distilled HGla                                                                                                                                                                             

3.   Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.

4.   Aspirate supernatant using sterile Pasteur pipet.

5.   Resuspend pellet gently in remaining supernatant.

6.   Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:

A.  Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.  Add the remaining 4 volumes of glycerolyte and gently agitate.

7.   Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).

8.   Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

       

Name of Depositor M Yoeli
Year of Origin 1965
References

Yoeli M, et al. Gametogony and sporogony in a strain of Plasmodium berghei preserved at low temperature. J. Parasitol. 49: 926-929, 1963. PubMed: 14084197

Yoeli M, et al. Plasmodium berghei: cyclical transmissions by experimentally infected Anopheles quadrimaculatus. Science 144: 1580-1581, 1964. PubMed: 14169345

Holbrook TW, et al. Glucan-enhanced immunogeneity of killed erythrocytic stages of Plasmodium berghei. Infect. Immun. 32: 542-546, 1981. PubMed: 7019074

Diggens SM, et al. Altered dihydrofolate reductase associated with a pyrimethamine- resistant Plasmodium berghei berghei produced in a single step. Nature 228: 579-580, 1970. PubMed: 5472487

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation