Trypanosoma lewisi (Kent) Laveran and Mesnil (ATCC® 30085)

Strain Designations: New Orleans-67  /  Depositor: RG Yaeger  /  Biosafety Level: 1

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Strain Designations New Orleans-67
Application
Vector borne research
Biosafety Level 1
Isolation
rat, Rattus norvegicus, New Orleans, 1967
Product Format frozen
Type Strain no
Comments
Flow cytometric analysis of immune-globulin and complement component C3 on the surface
Sensitivity to alternate complement pathway-mediated lysis
use of plastic ampoules for freeze preservation
Growth Conditions
Duration: in vivo, laboratory rat
Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Subcultivation
Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Name of Depositor RG Yaeger
Year of Origin 1967
References

Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Sturtevant JE, Balber AE. Trypanosoma lewisi: restriction of alternative complement pathway C3/C5 convertase activity. Exp. Parasitol. 63: 260-271, 1987. PubMed: 3556206

Sturtevant JE, Balber AE. Flow cytometric analysis of immunoglobulin and complement component C3 on the surface of Trypanosoma lewisi. J. Protozool. 33: 197-203, 1986. PubMed: 3525820

Sturtevant JE, Balber AE. Externally disposed membrane polypeptides of intact and protease- treated Trypanosoma lewisi correlated with sensitivity to alternate complement pathway-mediated lysis. Infect. Immun. 42: 869-875, 1983. PubMed: 6358042

Cross References

Nucleotide (GenBank) : AJ223566 Trypanosoma lewisi, small subunit ribosomal RNA gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation