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Prototheca wickerhamii Tubaki and Soneda (ATCC^® 16523^™)

Organism: Prototheca wickerhamii Tubaki and Soneda / Depositor: WB Cooke

Prototheca wickerhamii Tubaki and Soneda ATCC^® 16523^™

freeze-dried

For-Profit: $222.00 Non-Profit: $185.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	PR-28 [UTEX 1437]
Biosafety Level	2
Isolation	bovine mastitis, Georgia, (?)
Product Format	freeze-dried
Type Strain	no

Medium	ATCC^® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions	Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
Subcultivation	Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.

Name of Depositor	WB Cooke
Chain of Custody	ATCC <<--WB Cooke<<--NRRL Y-2464
References	Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS