SU-DHL-10 (ATCC® CRL-2963™)

Organism: Homo sapiens, human  /  Tissue: peritoneal effusion  /  Cell Type: B lymphocyte

Organism Homo sapiens, human
Tissue peritoneal effusion
Cell Type B lymphocyte
Morphology lymphoblast-like
Culture Properties suspension, multicellular aggregates
Biosafety Level 1
Disease Large Cell Lymphoma
Age 25 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data 25 years
Caucasian
Male
Comments ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Protocol: Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 2.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.
Medium renewal: every 2 to 4 days
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 11, 12
D13S317: 10, 11
D7S820: 8, 11
D16S539: 10, 11
vWA: 17
THO1: 8, 9.3
TPOX: 8
CSF1PO: 10, 13
Amelogenin: XY
Isotype IgG lambda
Name of Depositor A Epstein
Year of Origin 1978