pJCF31 [pJCF22.H51] (ATCC® 86981)

Applications: contains sequence DNA Segmentshuttle vector  /  Depositors: JG Cobley

Permits and Restrictions

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Designations pJCF31 [pJCF22.H51]
Depositors JG Cobley
Biosafety Level 1
Vector Information
Size (kb): 12.1000003814697300
Vector: pJCF31 (plasmid)
Construction: pJCF22, pFDA ori
Marker(s):cmlR
Construct size (kb): 12.10000038146973
Features: marker(s): cmlR
replicon: pMB1, pFDA
Applications
contains sequence DNA Segment
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--8.0, 4.1; BamHI--uncut; HindIII--3.9, 2.3, 2.1, 1.9, 1.0, 0.9.
Can be mobilized into F. diplosiphon using the conjugative plasmid RP4.
Shuttle vector.
A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid.
Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the ClaI site of pJCF22.
The order of the major features of the plasmid is: cmlR - bom - oriV - F. diplosiphon pFDA ori.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056

Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495

John G Cobley, personal communication

Shipped freeze-dried
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