The THLE-2 (ATCC CRL-10149)
and the THLE-3 (ATCC CRL-11233)
cell lines were derived from primary normal liver cells by infection with SV40 large T antigen. [RF84749] The virus was generated by introducing a retroviral vector containing the of Bgl I-Hpa I fragment of SV40 T antigen into the amphotropic packaging cell line PA317.
THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells.
They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750]
THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways. [RF84750]
Other enzymes involved in metabolism of chemical carcinogens, such as epoxide hydrolase, NADPH cytochrome P450 reductase, superoxide dismutase, catalase, glutathione S-transferases, and glutathione peroxidase are also retained by THLE cells. [RF84750]
A culture submitted to the ATCC in May of 1989 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. The cured cell line is available as CRL-2706. The original patent deposit is available as CRL-10149