Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43255)

Strain Designations: VPI 10463  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations VPI 10463
Application
Enteric Research
Isolation
Abdominal wound
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Images Micrograph of Cells at 1000X ATCC 43255
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxinotype 0
Binary toxin gene cdtB not amplified by PCR
Ribotype 087
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Medium Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2  or 80% N2-20% CO2
Genotype Toxinotype: 0
Name of Depositor TD Wilkins, DM Lyerly
Chain of Custody
ATCC <-- TD Wilkins, DM Lyerly <-- G Nygaard
Isolation
Abdominal wound
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

BD GeneOhm Cdiff. BD Becton Dickinson.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084