YRp7-32phocat (ATCC® 37625)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YRp7-32phocat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 6.5300002098083500
Vector: YRp7-32phocat (plasmid)
Promoters: Promoter PHO5
Construction: YRp7-32cat (ATCC 37624), PHO5
Marker(s):TRP1,ampR
Construct size (kb): 6.53000020980835
Features: marker(s): ampR, TRP1
promoter: PHO5
replicon: pMB1, ARS1
Applications
YR-type (replicating) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.37, 0.55; PstI--5.5, 1.3; PvuII--4.7, 2.15.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 570 bp SalI/EcoRI fragment containing the PHO5 promoter was ligated to a 5965 bp BamHI/SalI fragment of YRp7-32cat (ATCC 37624).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

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Shipping Information Distributed: freeze-dried
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