CCD-1097Sk (ATCC® CRL-2124)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Carcinoma

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinoma
Age 31 years
Gender female
Ethnicity Caucasian
Applications
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Derivation
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Clinical Data
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
female
Caucasian
31 years
Comments
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Passage History
Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings.