pSP1 (ATCC® 77497)

Applications: shuttle vectorvector containing primer sites useful for sequencingvector for generating nested deletions of cloned insertsvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: G Cottarel

Designations pSP1
Depositors G Cottarel
Biosafety Level 1
Vector Information
Size (kb): 6.7030000686645510
Vector: pSP1 (phagemid)
Promoters: Promoter T3
Construction: pRS305 (ATCC 77140)
Marker(s):LEU2,ampR
Construct size (kb): 6.703000068664551
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac, T3, T7
replicon: ars1
replicon: f1
replicon: pMB1
Applications
shuttle vector
vector containing primer sites useful for sequencing
vector for generating nested deletions of cloned inserts
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--5.0, 1.8; SstI--6.7; HindIII--6.7.
Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation.
Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed.
The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314

Related Products
component of:ATCC 87686
Shipped frozen
Shipping Information Glycerol stock of E. coli containing the plasmid
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