WI-38 VA-13 subline 2RA (ATCC® CCL-75.1)

Organism: Homo sapiens, human  /  Cell Type: fibroblast (SV40 transformed)  /  Tissue: lung  / 

Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast (SV40 transformed)
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain polyomavirus DNA sequences]
Age 3 months gestation
Gender female
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 76; range = 45 to 89.
Karyotype unstable within hyperdiploid stemline range 73-78. Ninety percent of the cells examined have 2 or 3 large chromosomes with subterminal centromeres and 1-6 microchromosomes. Secondary constrictions were observed in 59% of the cells, 21% had breaks.
Clinical Data
3 months gestation
Caucasian
female
Virus Susceptibility Human poliovirus 2
Herpes simplex virus
Vesicular stomatitis virus
Comments

Note:  Cytogenetic information is based on initial seed stock at ATCC.  Cytogenetic instability has been reported in the literature for some cell lines.

This cell line is susceptible to infection by the following viruses: herpes simplex; vesicular stomatitis (Indiana); poliovirus 2

This cell line is negative for reverse transcriptase.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

    Medium Renewal: Twice per week
    Cryopreservation
    Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,12
    D13S317: 11
    D16S539: 11,12
    D5S818: 10
    D7S820: 9,11
    THO1: 9.3
    TPOX: 8
    vWA: 19,20
    Isoenzymes
    G6PD, B
    Name of Depositor AJ Girardi
    Year of Origin March 1964
    References

    Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661

    Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

    Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963

    Basic Documentation
    References

    Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661

    Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

    Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963