NCEB-1 ATCC ® CRL-3005™ Homo sapiens; Mus musculus lymphoma

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens; Mus musculus, human; mouse
Cell Type	lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transforme
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2
Disease	lymphoma
Age	57 year old ( human)
Applications	ATCC cautions scientist on the use of the NCEB-1 cell line as an in vitro model for the study of Mantle Cell Lymphoma. Cytogenetic analysis of the cell line demonstrated two clones with variations: a hypodiploid clone, with a complex karyotype including a t(11;14)(q13;q32) similar to the original tumor cells, and a near tetraploid clone with the same markers. This finding at ATCC is based on karyotyping analysis (G-banding). NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda.
Storage Conditions	Liquid nitrogen vapor phase

Karyotype	This is a near tetraploid human/mouse hybrid cell line with a modal chromosome number of 94 and a low polyploidy rate. Most of the derivative chromosomes described in the original cell line, including the t(11;14)(q13;q32) marker are still present. All of the examined cells of this cell line contain several intact mouse chromosomes.
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Derivation	NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda. Cytogenetic analysis of the cell line demonstrated two clones with variations: a hypodiploid clone, with a complex karyotype including a t(11;14)(q13;q32) similar to the original tumor cells, and a near tetraploid clone with the same markers. The cell line was not tumorigenic when tested in an in vitro assay using immunosuppressed mice. [PubMed: 2848599]. It was reported in 2006 that surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. [PubMed: 16448697]. ATCC cautions scientist on the use of the NCEB-1 cell line as an in vitro model for the study of Mantle Cell Lymphoma. Published data from researchers and testing performed during the ATCC accessioning process have confirmed that this cell line is a human-mouse hybrid. This finding at ATCC is based on karyotyping analysis (G-banding).
Clinical Data	NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda.
Antigen Expression	CD3-, CD5+, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Genes Expressed	CD3-, CD5+, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Comments	NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda. Cytogenetic analysis of the cell line demonstrated two clones with variations: a hypodiploid clone, with a complex karyotype including a t(11;14)(q13;q32) similar to the original tumor cells, and a near tetraploid clone with the same markers. The cell line was not tumorigenic when tested in an in vitro assay using immunosuppressed mice. [PubMed: 2848599]. It was reported in 2006 that surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. [PubMed: 16448697]. ATCC cautions scientist on the use of the NCEB-1 cell line as an in vitro model for the study of Mantle Cell Lymphoma. Published data from researchers and testing performed during the ATCC accessioning process have confirmed that this cell line is a human-mouse hybrid. This finding at ATCC is based on karyotyping analysis (G-banding).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing	Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 2 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1.2 X 10(6) cells/ml. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10% strong>Storage temperature: liquid nitrogen vapor phase

STR Profile	Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 9,10 D5S818: 9,11 D7S820: 10,11 THO1: 6,9.3 TPOX: 11 vWA: 14,18
Population Doubling Time	about 19 hours

Name of Depositor	E. Campo
Year of Origin	1987
References	Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 Saltman, D.L., et al. Characterization of a new non-Hodgkin's lymphoma cell line (NCEB-1) with a chromosomal (11:14) translocation [t(11:14)(q13;q32)]. Blood.;72(6):2026-2030 (1988). PubMed: 2848599

Name of Depositor

E. Campo

Year of Origin

1987

References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Saltman, D.L., et al. Characterization of a new non-Hodgkin's lymphoma cell line (NCEB-1) with a chromosomal (11:14) translocation [t(11:14)(q13;q32)]. Blood.;72(6):2026-2030 (1988). PubMed: 2848599

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Characterization Data Cell Micrograph
References	Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 Saltman, D.L., et al. Characterization of a new non-Hodgkin's lymphoma cell line (NCEB-1) with a chromosomal (11:14) translocation [t(11:14)(q13;q32)]. Blood.;72(6):2026-2030 (1988). PubMed: 2848599

NCEB-1 (ATCC® CRL-3005™)

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NCEB-1 (ATCC^® CRL-3005^™)