MEF (CF-1) MITC (ATCC® SCRC-1040.2a)

Organism: Mus musculus, mouse  /  Cell Type: Fibroblast  /  Tissue: Embryo  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue Embryo
Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1
Age 14 days gestation embryo
Gender Male and female mixed
Strain CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
Applications
Mitomycin-C treated cells for use as feeder layer
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male and female mixed
Comments
These cells are provided for use as feeder cells to support the growth of stem cells in the undifferentiated state. They have been treated with Mitomycin C at passage #3 and will not replicate. The cells will begin to deteriorate 7 days after plating. 
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing

    Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 6 X 104 cells/cm2 in order to obtain a supportive monolayer for stem cell growth.

    Medium Renewal: Twice a week or when pH decreases
    Cryopreservation
    Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor ATCC
    Passage History
    These cells have been treated with Mitomycin C at passage #3 and will not replicate.
    Year of Origin 2004
    References

    Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.