Homo sapiens (ATCC® CRL-8296)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Subcultivation
Medium Renewal: Every 2 to 3 days
Agent 6T-CEM [derivative of CCRF-CEM (ATCC CCL-119)]
Strain Designations 6T-CEM [derivative of CCRF-CEM (ATCC CCL-119)]
Morphology lymphoblast
Age 4 years
Applications
This line may be used as a fusion partner for making human T cell hybridomas.
Application
This line may be used as a fusion partner for making human T cell hybridomas.
Gender female
Ethnicity Caucasian
Type Strain no
Biosafety Level 2
Comments
This is a 6-thioguanine resistant variant of CCRF-CEM.
It is deficient in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and secretes high titers of an immunosuppressive factor.
This line may be used as a fusion partner for making human T cell hybridomas.
suspension
immunosuppressive factor
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Product Format frozen
Designation / Description 6T-CEM Acute lymphocytic leukemia; Human
U.S. Patent Number
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation