pHPV-16 plasmid in E. coli (ATCC® 45113)

Organism: human papilloma virus type 16  /  Clone Type: Clone  /  Depositors: JE Marich, Syngene, Inc.

Permits and Restrictions

View Permits

Designations pHPV-16 plasmid in E. coli
GenBank Definition

Human papillomavirus type 16 (HPV16), complete genome

GenBank Number

K02718

Species human papilloma virus type 16
Depositors JE Marich, Syngene, Inc.
Vector Size of Construct (kb): 10.866
Excise insert: BamHI+BglI (insert fragment will include 205 nt of the vector).

Vector information:
Vector: pBluescript SK-
Size of intact vector (kb): 2.960
Markers: ampR
Vector ends: XhoI
Insert Insert size (kb): 7.904
Genome: human papilloma virus type 16
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: genomic
Insert ends: BamHI
Insert Size (kb) 7.904
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Purified plasmid DNA: ATCC 45113D
Biosafety Level 2
Shipping Information Frozen Escherichia coli DH5α containing the plasmid.
Comments
Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site
Full-length.
The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI - 685 [6818], 1320 [7453].
BamHI+BglI will excise the insert with 205 nucleotides of the vector.
Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6.
Classification Papovavirus, Papillomavirus, recombinant
References

James E Marich, personal communication

Product Sheet
Product Sheet
Product Sheet