QM7 (Quail muscle clone 7) (ATCC® CRL-1962)

Organism: Coturnix coturnix japonica, quail, Japanese  /  Cell Type: myoblast myoblast; chemically induce  /  Disease: fibrosarcoma

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Organism Coturnix coturnix japonica, quail, Japanese
Cell Type myoblast myoblast; chemically induce
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease fibrosarcoma
Age 1 to 3 weeks
Applications
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al.
In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin.
QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression.
To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells.
Derivation
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene.
Genes Expressed
desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form)
Cellular Products
desmin; cardiac troponin T; cardiac troponin C; skeletal troponin T; skeletal troponin I; alpha tropomyosin; muscle creatine kinase; myosin light chain 2; myosin heavy chain (ventricular form)
Comments
The QM7 cell line was derived from the QT6 fibrosarcoma originally isolated by Moscovici, et al. from a tumor that developed in a bird treated with methylcholanthrene.
QM7 is a serum inducible myogenic cell line.
The cells replicate as myoblasts in medium containing serum.
When switched to medium without serum, the cells cease dividing and fuse to form large multinucleated myotubes.
In the myotube state, the cells express muscle specific proteins such as desmin, cardiac troponin T, cardiac troponin C, skeletal troponin T, skeletal troponin I, alpha tropomyosin.
Mucle creatine kinase, myosin light chain 2 and a ventricular form of myosin heavy chain.
They do not express any known form of alpha actin.
QM7 cells transfect with high efficiency, and are useful for studying many aspects of muscle differentiation and gene expression.
The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cell, cultures should be subcultured before they become confluent and the line should be recloned periodically with selection for myoblastic cells.
Complete Growth Medium Medium 199 with Earle's BSS, 80%; tryptose phosphate broth, 10%; fetal bovine serum, 10%
Subculturing
Protocol: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent. Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin solution. Incubate the flask at room temperature until the cells detach (about 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Name of Depositor PB Antin
References

Antin PB, Ordahl CP. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143: 111-121, 1991. PubMed: 1985013

Rong S, Sheppard MG. Processes for preparation of Marek's disease virus using continuous avian cell lines. US Patent 6,410,297 dated Jun 25 2002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Antin PB, Ordahl CP. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143: 111-121, 1991. PubMed: 1985013

Rong S, Sheppard MG. Processes for preparation of Marek's disease virus using continuous avian cell lines. US Patent 6,410,297 dated Jun 25 2002