pKSM712 (ATCC® 87158)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM712
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.7409999370574950
Vector: pKSM712 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC4-KIXX
Marker(s):kanR
Construct size (kb): 3.740999937057495
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: BamHI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
Applications
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.75; EcoRI--3.75; PstI--3.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--2.89; EcoRI--0.39; NcoI--0.33, 3.49; PstI--3.11; PvuI--0.51; PvuII--0.48, 2.80, 3.16.
Differs from pKSM711 (ATCC 87157) only in the orientation of the kanamycin resistance gene.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map