NCTC 3749 (ATCC® CCL-46.1)

Organism: Mus musculus, mouse  /  Disease: lymphoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain DBA/2
Applications
When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS).
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line is a derivative of P388D1 (ATCC CCL-46).
Antigen Expression
H-2d
Genes Expressed
produce interleukin-1 (IL-1, interleukin 1),H-2d
Cellular Products
produce interleukin-1 (IL-1, interleukin 1)
Virus Resistance
poliovirus 1
Comments
This line is a derivative of P388D1 (ATCC CCL-46).
When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium NCTC 135 medium, 100%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove one-half of the old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor VJ Evans
References

Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171

Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957

Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094

. . Nature 181: 1343, 1958.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171

Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957

Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094

. . Nature 181: 1343, 1958.