55-6 (ATCC® CRL-2156)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB and weakly to gp120 MN.
It binds to the C1 region (positions 105 - 117) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
55-6 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
55-6 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB and weakly to gp120 MN.
It binds to the C1 region (positions 105 - 117) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    Culture medium, 95%; DMSO, 5%
    Isotype IgG2a
    Name of Depositor SL Epstein
    References

    Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785